Assay of omeprazole and omeprazole sulfone by semi-microcolumn liquid chromatography with mixed-function precolumn

Citation
Ds. Yim et al., Assay of omeprazole and omeprazole sulfone by semi-microcolumn liquid chromatography with mixed-function precolumn, J CHROMAT B, 754(2), 2001, pp. 487-493
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
754
Issue
2
Year of publication
2001
Pages
487 - 493
Database
ISI
SICI code
1387-2273(20010425)754:2<487:AOOAOS>2.0.ZU;2-1
Abstract
A column-switching system based on semi-microcolumns was used for direct an alysis of omeprazole and omeprazole sulfone in human plasma samples. Plasma samples were injected into a mixed-function (MF Ph-1) column (35 mmx4.6 mm I.D.) to remove proteins and other non-specific peak producing substances from the analyte-containing time zone. The analyte-containing fraction was thereafter transferred to a C-18 semi-microcolumn (250 mmx1.5 mm I.D.) afte r concentration at the C-18 intermediate column. The absorbance at 302 nm i n a ultraviolet (UV) detector was recorded to measure the concentration. Th e detection limit for omeprazole and omeprazole sulfone in the present meth od was 10 ng/ml. Interbatch variation (coefficient of variation) of the QC samples spanned less than 10% and intra-batch variation less than 2%. The r ecovery ratios of omeprazole and omeprazole sulfone were over 98%. The curr ent method can be used as a simpler procedure with similar sensitivity and reproducibility as previously reported methods. (C) 2001 Elsevier Science B .V. All rights reserved.