Characterization of aryl alcohol oxidase produced by dye-decolorizing fungus, Geotrichum candidum Dec1

Citation
Sj. Kim et al., Characterization of aryl alcohol oxidase produced by dye-decolorizing fungus, Geotrichum candidum Dec1, J BIOSCI BI, 91(2), 2001, pp. 166-172
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
91
Issue
2
Year of publication
2001
Pages
166 - 172
Database
ISI
SICI code
1389-1723(200102)91:2<166:COAAOP>2.0.ZU;2-8
Abstract
Aryl alcohol oxidase (AAO) produced by Geotrichum candidum Dec1(Dec1), a ne wly isolated decolorizing fungus, was purified by ultrafiltration and by us ing diethylaminoethyl (DEAE) Sephacel, Butyl-Toyopearl and Mono-Q columns. H2O2 produced by concomitant AAO oxidation of veratryl alcohol (VA) to vera traldehyde was consumed by a peroxidase (DyP) purified from Dec1 culture, l eading to the decolorization of a dye in vitro. In the liquid culture of De c1, the existence of H2O2 and veratraldehyde was confirmed during cultivati on, when dye-decolorization and AAO activities were maintained. This indica tes that VA produced by Dec1 was oxidized by AAO to veratraldehyde, generat ing H2O2, which supported dye-decolorizing activity of Dec1 in vivo. The pr evention of polymerization of DyP oxidation products of a dye in the presen ce of AAO was shown.