Stereoselective oxidation of alkylbenzenes by fungi

Citation
A. Uzura et al., Stereoselective oxidation of alkylbenzenes by fungi, J BIOSCI BI, 91(2), 2001, pp. 217-221
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
91
Issue
2
Year of publication
2001
Pages
217 - 221
Database
ISI
SICI code
1389-1723(200102)91:2<217:SOOABF>2.0.ZU;2-Y
Abstract
Oxygenase is useful when oxygen Is to be introduced at a nonactivated carbo n-hydrogen bond to give an optically active center. To obtain such an enzym e from microorganisms, we screened soil samples for organisms that assimila ted methylethylketone as their sole carbon source. Yeasts and molds that co nverted ethylbenzene and propylbenzene into their respective oxygenated pro ducts during incubation together as resting cells were isolated. One partic ularly potent strain was identified as Fusarium moniliforme. The fungus oxi dized the side chains of ethylbenzene and propylbenzene selectively at the benzylic position. The products were found to be 1-phenylethanol and 1-phen ylpropanol, respectively, by CC-MS and HPLC with a chiral column, with 100% enantiomeric excess of the (R)-(+)-form.