Preparation of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate in an anaerobic interface bioreactor

Citation
S. Oda et al., Preparation of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate in an anaerobic interface bioreactor, J BIOSCI BI, 91(2), 2001, pp. 202-207
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
91
Issue
2
Year of publication
2001
Pages
202 - 207
Database
ISI
SICI code
1389-1723(200102)91:2<202:POMUVM>2.0.ZU;2-K
Abstract
An interface bioreactor, which is a device for the microbial transformation of water-insoluble substrates, was applied to an anaerobic bioconversion f or the first time. Methyl 7-ketolithocholate [Me-7KLCA] was reduced with th e human intestinal bacterium Eubacterium aerofaciens JCM 7790 in a convenie nt anaerobic interface bioreactor using a nutrient agar plate placed in a G asPak (TM) pouch. The resulting methyl ursodeoxycholate [Me-UDCA] is a prec ursor of ursodeoxycholic acid, which is used as a cholesterol gallstone-dis solving agent. The reaction conditions were optimized, and ABCM medium and dihexyl ether were selected as the best carrier and reaction solvent, respe ctively. The toxicity of the bile acid esters toward the human intestinal b acterium was effectively alleviated in the interface bioreactor, in which t he maximal concentrations of Me7KLCA and Me-UDCA in the dihexyl ether layer respectively reached to 12.0 and 6.1 g/l.