Synthesis of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate with Eubacterium aerofaciens JCM 7790 grown on two kinds ofcarbon and hydride sources, glucose and mannitol

Citation
S. Oda et al., Synthesis of methyl ursodeoxycholate via microbial reduction of methyl 7-ketolithocholate with Eubacterium aerofaciens JCM 7790 grown on two kinds ofcarbon and hydride sources, glucose and mannitol, J BIOSCI BI, 91(2), 2001, pp. 178-183
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
91
Issue
2
Year of publication
2001
Pages
178 - 183
Database
ISI
SICI code
1389-1723(200102)91:2<178:SOMUVM>2.0.ZU;2-S
Abstract
Eubacterium aerofaciens JCM 7790, which produces 7 beta -hydroxysteroid deh ydrogenase [7 beta -HSDH], efficiently catalyzed the reduction of methyl 7- ketolithocholate [Me-7KLCA] to afford methyl ursodeoxycholate [Me-UDCA] in an anaerobic interface bioreactor. For the high accumulation of Me-UDCA, th e best supplement of carbon and hydride source to the ABCM medium (glucose content, 0.3 wt.%) was screened because glucose strongly repressed 7 beta - HSDH production. The supplementation of cellobiose, mannitol, sodium glucon ate, and sodium pyruvate was effective to improve the production of Me-UDCA , although many mono-and disaccharides, such as fructose, sucrose, and lact ose, strongly repressed 7 beta -HSDH activity. A phosphate buffer was used as the aqueous phase in the carrier to avoid the lowering of medium pH in t he carrier caused by the accumulation of organic acids via fermentation by E. aerofaciens. Thus, the productivity of Me-UDCA was elevated by the combi nation of glucose and mannitol as carbon and hydride sources and by using a phosphate buffer.