Glycolaldehyde-forming route in Bacillus subtilis in relation to vitamin B-6 biosynthesis

Citation
A. Sakai et al., Glycolaldehyde-forming route in Bacillus subtilis in relation to vitamin B-6 biosynthesis, J BIOSCI BI, 91(2), 2001, pp. 147-152
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
91
Issue
2
Year of publication
2001
Pages
147 - 152
Database
ISI
SICI code
1389-1723(200102)91:2<147:GRIBSI>2.0.ZU;2-6
Abstract
Glycolaldehyde (GA) was shown to be a precursor of vitamin B-6 (B-6), and t o be formed from glycolate by glycolaldehyde de hydrogenase (GADH) in Esche richia coli. In th is study, we show the glycolaldehyde-forming route in B, biosynthesis in Bacillus subtilis. In the crude extract of B. subtilis, th e oxidizing activity of GADH was detected. However, coexisting NADH/NADPH o xidase activity interfered with the determination of the reducing (GA-formi ng) activity of GADH. NADH/NADPH oxidase was purified and identified as the product of ahpF. In an ahpF disruptant, NADH/NADPH activity was almost eli minated, but the reducing activity of GADH was not detected. We also invest igated another possible GA-forming enzyme, glyoxal reductase (GR). GR was p artially purified and identified as the product of yvgN. yvgN disruptant di d not require B-6, and retained the ability to synthesize the same amount o f B-6 as the wild-type strain. From these results, we concluded that neithe r GADH nor GR is involved in B-6 biosynthesis in B. subtilis.