Interplay between the specific chaperone-like proteins HybG and HypC in maturation of hydrogenases 1, 2, and 3 from Escherichia coli

Citation
M. Blokesch et al., Interplay between the specific chaperone-like proteins HybG and HypC in maturation of hydrogenases 1, 2, and 3 from Escherichia coli, J BACT, 183(9), 2001, pp. 2817-2822
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
183
Issue
9
Year of publication
2001
Pages
2817 - 2822
Database
ISI
SICI code
0021-9193(200105)183:9<2817:IBTSCP>2.0.ZU;2-Q
Abstract
The hybG gene product from Escherichia coli has been identified as a chaper one like protein acting in the maturation of hydrogenases 1 and 2, It was s hown that HybG forms a complex with the precursor of the large subunit of h ydrogenase 2, As with HypC, which is the chaperone-like protein involved in hydrogenase 3 maturation, the N-terminal cysteine residue is crucial for c omplex formation. Introduction of a deletion into hybG abolished the genera tion of active hydrogenase 2 but only quantitatively reduced hydrogenase 1 activity since HypC could replace HybG in this function. In contrast, HybG could not take over the role of HypC in a Delta hypC genetic background. Ov erproduction of HybG, especially of the variants with the replaced N-termin al cysteine residue, strongly interfered with hydrogenase 3 maturation, app arently by titrating some other component(s) of the maturation machinery. T he results indicate that the three hydrogenase isoenzymes not only are inte racting at the functional level but are also interconnected during the matu ration process.