Growth phase variation in cell and nucleoid morphology in a Bacillus subtilis recA mutant

Citation
Sa. Sciochetti et al., Growth phase variation in cell and nucleoid morphology in a Bacillus subtilis recA mutant, J BACT, 183(9), 2001, pp. 2963-2968
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
183
Issue
9
Year of publication
2001
Pages
2963 - 2968
Database
ISI
SICI code
0021-9193(200105)183:9<2963:GPVICA>2.0.ZU;2-A
Abstract
The major role of RecA is thought to be in helping repair and restart stall ed replication forks. During exponential growth, Bacillus subtilis recA cel ls exhibited few microscopically observable nucleoid defects. However, the efficiency of plating was about 12% of that of the parent strain. A substan tial and additive defect in viability was also seen for addB and recF mutan ts, suggesting a role for the corresponding recombination paths during norm al growth. Upon entry into stationary phase, a subpopulation (similar to 15 %) of abnormally long cells and nucleoids developed in B. subtilis recA mut ants. In addition, recA mutants showed a delay in, and a diminished capacit y for, effecting prespore nucleoid condensation.