Bap, a Staphylococcus aureus surface protein involved in biofilm formation

Citation
C. Cucarella et al., Bap, a Staphylococcus aureus surface protein involved in biofilm formation, J BACT, 183(9), 2001, pp. 2888-2896
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
183
Issue
9
Year of publication
2001
Pages
2888 - 2896
Database
ISI
SICI code
0021-9193(200105)183:9<2888:BASASP>2.0.ZU;2-G
Abstract
Identification of new genes involved in biofilm formation is needed to unde rstand the molecular basis of strain variation and the pathogenic mechanism s implicated in chronic staphylococcal infections. A biofilm-producing Stap hylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion mutants, Two mutants were found with a significant decrea se in attachment to inert surfaces (early adherence), intercellular adhesio n, and biofilm formation. The transposon was inserted at the same locus in both mutants. This locus (bap [for biofilm associated protein] encodes a no vel cell wall associated protein of 2,276 amino acids (Bap), which shows gl obal organizational similarities to surface proteins of gram-negative (Pseu domonas aeruginosa and Salmonella enterica serovar Typhi) and gram-positive (Enteroccocus faecalis) microorganisms. Bap's core region represents 52% o f the protein and consists of 13 successive nearly identical repeats, each containing 86 amino acids. bap was present in a small fraction of bovine ma stitis isolates (5% of the 350 S. aureus isolates tested), but it was absen t from the 75 clinical human S. aureus isolates analyzed. All staphylococca l isolates harboring bap were highly adherent and strong biofilm producers. In a mouse infection model bap was involved in pathogenesis, causing a per sistent infection.