Specific binding of integrase to the origin of transfer (oriT) of the conjugative transposon Tn916

Citation
D. Hinerfeld et G. Churchward, Specific binding of integrase to the origin of transfer (oriT) of the conjugative transposon Tn916, J BACT, 183(9), 2001, pp. 2947-2951
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
183
Issue
9
Year of publication
2001
Pages
2947 - 2951
Database
ISI
SICI code
0021-9193(200105)183:9<2947:SBOITT>2.0.ZU;2-C
Abstract
Purified integrase protein (Int) of the conjugative transposon Tn916 was sh own, using nuclease protection experiments, to bind specifically to a site within the origin of conjugal transfer of the transposon, oriT. A sequence similar to the ends of the transposon that are bound by the C-terminal DNA- binding domain of Int was present in the protected region. However, Int bin ding to oriT required both the N- and C-terminal DNA-binding domains of Int , and the pattern of nuclease protection differed from that observed when I nt binds to the transposon ends and flanking DNA. Binding of Int to oriT ma y be part of a mechanism to prevent premature conjugal transfer of Tn916 pr ior to excision from the donor DNA.