Low-molecular-weight plasmid of Salmonella enterica serovar enteritidis codes for retron reverse transcriptase and influences phage resistance

Citation
I. Rychlik et al., Low-molecular-weight plasmid of Salmonella enterica serovar enteritidis codes for retron reverse transcriptase and influences phage resistance, J BACT, 183(9), 2001, pp. 2852-2858
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
183
Issue
9
Year of publication
2001
Pages
2852 - 2858
Database
ISI
SICI code
0021-9193(200105)183:9<2852:LPOSES>2.0.ZU;2-5
Abstract
Retron reverse transcriptases are unusual procaryotic enzymes capable of sy nthesis of low-molecular-weight DNA by reverse transcription. All of the so -far-described DNA species synthesized by retron reverse transcriptases hav e been identified as multicopy single-stranded DNA. We have shown that Salm onella enterica serovar Enteritidis is also capable of synthesis of the low -molecular-weight DNA by retron reverse transcriptase. Surprisingly, Salmon ella serovar Enteritidis-produced low-molecular-weight DNA was shown to be a double-stranded DNA with single-stranded overhangs (sdsDNA). The sdsDNA w as 72 nucleotides (nt) long, of which a 38-nt sequence was formed by double -stranded DNA with 1.9- and 15-nt single-stranded overhangs, respectively. Three open reading frames (ORFs), encoded by the 4,053-bp plasmid, were ess ential for the production of sdsDNA. These included an ORF with an unknown function, the retron reverse transcriptase, and an ORF encoding the cold sh ock protein homologue. This plasmid was also able to confer phage resistanc e onto the host cell by a mechanism which was independent of sdsDNA synthes is.