Mapping of the Rsd contact site on the sigma 70 subunit of Escherichia coli RNA polymerase

Citation
M. Jishage et al., Mapping of the Rsd contact site on the sigma 70 subunit of Escherichia coli RNA polymerase, J BACT, 183(9), 2001, pp. 2952-2956
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
183
Issue
9
Year of publication
2001
Pages
2952 - 2956
Database
ISI
SICI code
0021-9193(200105)183:9<2952:MOTRCS>2.0.ZU;2-6
Abstract
Rsd (regulator of sigma D) is an anti-sigma factor for the Escherichia coli RNA polymerase sigma (70) subunit. The contact site of Rsd on sigma (70) w as analyzed after mapping of the contact-dependent cleavage sites by Rsd-te thered iron-p-bromoacetamidobenzyl EDTA and by analysis of the complex form ation between Ala-substituted sigma (70) and Rsd. Results indicate that the Rsd contact site is located downstream of the promoter -35 recognition hel ix-turn-helix motif within region 4, overlapping with the regions involved in interaction with both core enzyme and sigma (70) contact transcription f actors.