Selective mRNA degradation by polynucleotide phosphorylase in cold shock adaptation in Escherichia coli

Citation
K. Yamanaka et M. Inouye, Selective mRNA degradation by polynucleotide phosphorylase in cold shock adaptation in Escherichia coli, J BACT, 183(9), 2001, pp. 2808-2816
Citations number
66
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
183
Issue
9
Year of publication
2001
Pages
2808 - 2816
Database
ISI
SICI code
0021-9193(200105)183:9<2808:SMDBPP>2.0.ZU;2-2
Abstract
Upon cold shock, Escherichia coli cell growth transiently stops. During thi s acclimation phase, specific cold shock proteins (CSPs) are highly induced . At the end of the acclimation phase, their synthesis is reduced to new ba sal levels, while the non-cold shock protein synthesis is resumed, resultin g in cell growth reinitiation. Here, we report that polynucleotide phosphor ylase (PNPase) is required to repress CSP production at the end of the accl imation phase. A pnp mutant, upon cold shock, maintained a high level of CS Ps even after 24 h. PNPase was found to be essential for selective degradat ion of CSP mRNAs at 15 degreesC. In a poly(A) polymerase mutant and a CsdA RNA helicase mutant, CSP expression upon cold shock was significantly prolo nged, indicating that PNPase in concert with poly(A) polymerase and CsdA RN A helicase plays a critical role in cold shock adaptation.