Wolbachia in the Asian rice gall midge, Orseolia oryzae (Wood-Mason): correlation between host mitotypes and infection status

Citation
Sk. Behura et al., Wolbachia in the Asian rice gall midge, Orseolia oryzae (Wood-Mason): correlation between host mitotypes and infection status, INSEC MOL B, 10(2), 2001, pp. 163-171
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Entomology/Pest Control","Molecular Biology & Genetics
Journal title
INSECT MOLECULAR BIOLOGY
ISSN journal
0962-1075 → ACNP
Volume
10
Issue
2
Year of publication
2001
Pages
163 - 171
Database
ISI
SICI code
0962-1075(200104)10:2<163:WITARG>2.0.ZU;2-T
Abstract
Using a PCR-based method, we detected Wolbachia in the Asian rice gall midg e, Furthermore, results showed that all females across all biotypes are inf ected with Wolbachia, However, all male flies are not Infected and show dif ferent Infection frequency in different biotypes. We have also identified t hree mitotypes, in the rice gall midge, based on Dral restriction pattern o f a portion of the 12S rRNA gene that was PCR amplified using primers speci fic to this gene. All the females and infected male flies had type 1 mtDNA while uninfected males showed only type 2 or 3 mtDNA, Inheritance patterns of mtDNA revealed the existence of a correlation between mtDNA type and Wol bachia infection in the Asian rice gall midge. Evidence for paternal inheri tance of mtDNA in Wolbachia-free gall midge is also presented.