Tumour suppressor activity of human imprinted gene PEG3 in a glioma cell line

Citation
T. Kohda et al., Tumour suppressor activity of human imprinted gene PEG3 in a glioma cell line, GENES CELLS, 6(3), 2001, pp. 237-247
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENES TO CELLS
ISSN journal
1356-9597 → ACNP
Volume
6
Issue
3
Year of publication
2001
Pages
237 - 247
Database
ISI
SICI code
1356-9597(200103)6:3<237:TSAOHI>2.0.ZU;2-G
Abstract
Background: Mouse imprinted gene Peg3 encodes a large C2H2 type zinc finger protein with unique characteristics. Peg3 knockout mice were found to show an impairment in maternal behaviour of the adult female. Mouse Peg3 is loc ated on the proximal region of chromosome 7 which is syntenic to the long a rm of human chromosome 19. It has been reported that a loss of heterozygosi ty (LOH) of chromosome 19q occurs frequently in several glioma types. Results: We isolated human PEG3 cDNA. Both human and mouse PEG3 were strong ly expressed in the adult brain and the Peg3 protein was localized in the n uclei of both neurones and glial cells. A significant decrease in PEG3 expr ession was more commonly observed in glioma cell lines as compared with tha t in primary cultures of astrocytes. Transfection of PEG3 cDNA in a glioma cell line resulted in a loss of tumorigenicity in nude mice. Conclusions: The human PEG3 gene is a paternally expressed imprinted gene. Introduction of PEG3 cDNA into the glioma cells suggests that human PEG3 pr otein functions as a tumour suppressor. Human PEG3 is located on 19q13.4 an d is one of the candidates for tumour suppressor genes that are predicted t o be sited in gliomas.