Application of gel microdroplet and flow cytometry techniques to selectiveenrichment of non-growing bacterial cells

Citation
A. Manome et al., Application of gel microdroplet and flow cytometry techniques to selectiveenrichment of non-growing bacterial cells, FEMS MICROB, 197(1), 2001, pp. 29-33
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
0378-1097 → ACNP
Volume
197
Issue
1
Year of publication
2001
Pages
29 - 33
Database
ISI
SICI code
0378-1097(20010401)197:1<29:AOGMAF>2.0.ZU;2-R
Abstract
We describe an application or gel microdroplet (GMD) and flow cytometry tec hniques to selective enrichment of non-growing Leuconostoc mesenteroides ce lls, which are well culturable on other media, from a mixture with Bacillus subtilis cells in nutrient broth. After encapsulating cells of the mixed p opulation within GMDs and a brief incubation in nutrient broth. the inabili ty of L. mesenteroides cells to form microcolonies within GMDs allowed thei r discrimination from B. subtilis cells. After staining the GMD mixture wit h 6-carboxyfluorescein diacetate, which showed no influence on cell viabili ty, the GMDs containing single cells of L. mesenteroides were selectively c ollected using flow cytometry sorting based on differences in fluorescence intensity. The cells of L. mesenteroides retained viability during the proc ess. (C) 1001 Federation of European Microbiological Societies. Published b y Elsevier Science B.V. All rights reserved.