Functional characterization of sphingolipid C4-hydroxylase genes from Arabidopsis thaliana

Citation
P. Sperling et al., Functional characterization of sphingolipid C4-hydroxylase genes from Arabidopsis thaliana, FEBS LETTER, 494(1-2), 2001, pp. 90-94
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
0014-5793 → ACNP
Volume
494
Issue
1-2
Year of publication
2001
Pages
90 - 94
Database
ISI
SICI code
0014-5793(20010406)494:1-2<90:FCOSCG>2.0.ZU;2-7
Abstract
In the genome of Arabidopsis thaliana, two genes were identified encoding i soenzymes for C4-hydroxylation of long chain bases (LCB) in plant sphingoli pids, Both predicted proteins consist of 258 amino acid residues (77% ident ity) which show sequence similarity to di-iron-binding enzymes, such as Sur 2p and Erg3p from yeast, involved in oxygen-dependent lipid modifications. Heterologous expression of these genes in a yeast sur2 Delta -null mutant l acking C4-LCB hydroxylation resulted in the formation of D-ribo-C-18- and - C-20-phytosphinganine. The identity and stereochemical configuration of the isolated trihydroxybases was confirmed by electrospray ionization-mass spe ctroscopy, gas-liquid chromatography-mass spectrometry and H-1-nuclear magn etic resonance spectroscopy, These results represent the first functional i dentification of SUR2 genes from plants as well as from any organism other than yeast. (C) 2001 Published by Elsevier Science B,V, on behalf of the Fe deration of European Biochemical Societies.