Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs

Citation
C. Li et al., Optimization of conditions for bacteriocin extraction in PEG/salt aqueous two-phase systems using statistical experimental designs, BIOTECH PR, 17(2), 2001, pp. 366-368
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY PROGRESS
ISSN journal
8756-7938 → ACNP
Volume
17
Issue
2
Year of publication
2001
Pages
366 - 368
Database
ISI
SICI code
8756-7938(200103/04)17:2<366:OOCFBE>2.0.ZU;2-L
Abstract
The bacteriocin nisin was extracted in PEG/salt aqueous two-phase systems ( ATPS) using the property that the systems can extract hydrophobic proteins. The concentrations of the phase-forming components, PEG 4000 and Na2SO4, w ere optimized for nisin recovery by means of statistical experimental desig ns, and it was found that they strongly influenced nisin recovery. The opti mal composition of ATPS was found to be 15.99% (w/w) PEG 4000 and 15.85% (w /w) Na2SO4 (pH 2), and the optimal ATPS allowed an 11.60% increase of nisin recovery compared to the standard method of nisin assay.