Scale-up studies on a defined medium process for pilot plant production ofillicicolin by Gliocladium roseum

Citation
B. Junker et al., Scale-up studies on a defined medium process for pilot plant production ofillicicolin by Gliocladium roseum, BIOTECH PR, 17(2), 2001, pp. 278-286
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY PROGRESS
ISSN journal
8756-7938 → ACNP
Volume
17
Issue
2
Year of publication
2001
Pages
278 - 286
Database
ISI
SICI code
8756-7938(200103/04)17:2<278:SSOADM>2.0.ZU;2-F
Abstract
Illicicolin was cultivated at the 600-L pilot scale for purposes of materia l generation and process development. The initial medium containing oat flo ur was difficult operationally as a result of excessive foaming during ster ilization, so a new defined medium process (with either glucose or sucrose as the carbon source), developed at the 23-L scale, was scaled up and impro ved for pilot scale needs. Pilot scale media development efforts focused on exploring the highest concentration of media (1.0 x to 3.0x) that could be cultivated at the pilot scale and not be limited by mixing or oxygen mass transfer. The process was scaled up successfully and peak titers improved 7 .5-fold, from about 200 mg/L in the initial complex medium to 1500 mg/L in the final defined medium.