Cyclodextrin glucanotransferase (CGTase) gene of Bacillus macerans was subc
loned down-stream of yeast ADH1 promoter and expressed in Saccharomyces cer
evisiae. Most of the CGTase expressed was in the extracellular medium with
a maximum activity of about 0.28 unit ml(-1) after 48 h cultivation. The re
combinant CGTase was secreted as an N-linked-glycosylated form and predomin
antly produced alpha -cyclodextrin from starch.