Expression of Bacillus macerans cyclodextrin glucanotransferase gene in Saccharomyces cerevisiae

Citation
Sw. Nam et al., Expression of Bacillus macerans cyclodextrin glucanotransferase gene in Saccharomyces cerevisiae, BIOTECH LET, 23(9), 2001, pp. 727-730
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
0141-5492 → ACNP
Volume
23
Issue
9
Year of publication
2001
Pages
727 - 730
Database
ISI
SICI code
0141-5492(200105)23:9<727:EOBMCG>2.0.ZU;2-M
Abstract
Cyclodextrin glucanotransferase (CGTase) gene of Bacillus macerans was subc loned down-stream of yeast ADH1 promoter and expressed in Saccharomyces cer evisiae. Most of the CGTase expressed was in the extracellular medium with a maximum activity of about 0.28 unit ml(-1) after 48 h cultivation. The re combinant CGTase was secreted as an N-linked-glycosylated form and predomin antly produced alpha -cyclodextrin from starch.