Validated HPLC method for quantifying permethrin in pharmaceutical formulations

Citation
E. Garcia et al., Validated HPLC method for quantifying permethrin in pharmaceutical formulations, J PHARM B, 24(5-6), 2001, pp. 999-1004
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN journal
0731-7085 → ACNP
Volume
24
Issue
5-6
Year of publication
2001
Pages
999 - 1004
Database
ISI
SICI code
0731-7085(200103)24:5-6<999:VHMFQP>2.0.ZU;2-V
Abstract
An isocratic HPLC method for permethrin determination in raw material and p harmaceutical presentations as lotion and shampoo has been developed and va lidated following ICH recommendations. Cis and trans-isomers, impurities an d degradation products are well separated. The chromatographic analysis wer e performed on a 4 mum particle C-18 Nova-Pak (Waters, Madrid, Spain) colum n (15 x 0.39 cm) kept in a Biorad column oven at 35 degreesC. Mobile phase consisted of methanol-water (78.22, v/v) at a how rate of 1 ml/min. UV dete ction was performed at 272 nm and peaks were identified with retention time s as compared with standards and confirmed with characteristic spectra usin g the photodiode array detector. (C) 2001 Elsevier Science B.V. All rights reserved.