Expression of vascular endothelial growth factor receptor 3 in blood and lymphatic vessels of lung adenocarcinoma

Citation
T. Niki et al., Expression of vascular endothelial growth factor receptor 3 in blood and lymphatic vessels of lung adenocarcinoma, J PATHOLOGY, 193(4), 2001, pp. 450-457
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF PATHOLOGY
ISSN journal
0022-3417 → ACNP
Volume
193
Issue
4
Year of publication
2001
Pages
450 - 457
Database
ISI
SICI code
0022-3417(200104)193:4<450:EOVEGF>2.0.ZU;2-X
Abstract
Vascular endothelial growth factor receptor 3 (VEGFR-3) has been proposed a s a marker for lymphatic endothelial cells. This study investigated the exp ression of VEGFR-3 in the tumour vessels of lung adenocarcinoma and evaluat ed whether VEGFR-3 staining was useful for identifying lymphatic vessels wi thin the tumo. It also explored whether active growth of lymphatic vessels occurred in lung adenocarcinoma. Formalin-fixed, paraffin-embedded specimen s obtained from 60 cases of lung adenocarcinoma, including five cases of pu re bronchiolo-alveolar carcinoma (BAC) without stromal, vascular, and pleur al invasion, were examined. No VEGFR-3-positive vessels were observed in pu re BAC, but varying numbers of VEGFR-3-positive vessels were found in 39 of 55 (70.9%) invasive adenocarcinomas. A comparison of serial sections stain ed for VEGFR-3, CD31, and laminin-1 showed that most of the VEGFR-3-positiv e vessels appeared to be blood vessels (CD31-positive, laminin-1-positive), but some had the characteristics of lymphatic vessels (variable staining f or CD31, little or no staining for laminin-1). VEGFR-3 staining highlighted lymphatic invasion by cancer tells; this invasion could not be detected by CD31 or haematoxylin and eosin (H&E) staining. Active growth of lymphatic vessels (as indicated by nuclear Ki-67 labelling of the endothelium) was ob served in five tumours, four of which showed a high level of lymphatic inva sion by cancer cells. It was concluded that VEGFR-3 immunostaining did not discriminate clearly between vascular and lymphatic endothelial cells, sinc e expression of VEGFR-3 can be up-regulated in tumour blood vessels. Howeve r, VEGFR-3 staining combined with laminin-1 and CD31 staining would be usef ul for identifying lymphatic vessels and their invasion by tumour cells in a more objective way. Finally, proliferation of lymphatic endothelial cells may occur in association with lymphatic invasion by cancer cells. Copyrigh t (C) 2001 John Wiley & Sons, Ltd.