Biological characterization and optical imaging of brain-derived neurotrophic factor-green fluorescent protein suggest an activity-dependent local release of brain-derived neurotrophic factor in neurites of cultured hippocampal neurons

Citation
M. Kojima et al., Biological characterization and optical imaging of brain-derived neurotrophic factor-green fluorescent protein suggest an activity-dependent local release of brain-derived neurotrophic factor in neurites of cultured hippocampal neurons, J NEUROSC R, 64(1), 2001, pp. 1-10
Citations number
52
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROSCIENCE RESEARCH
ISSN journal
0360-4012 → ACNP
Volume
64
Issue
1
Year of publication
2001
Pages
1 - 10
Database
ISI
SICI code
0360-4012(20010401)64:1<1:BCAOIO>2.0.ZU;2-T
Abstract
To visualize the release dynamics of the brain-derived neurotrophic factor (BDNF) involved in neural plasticity, we constructed a plasmid encoding gre en fluorescent protein (GFP) fused with BDNF. First, several biological stu dies confirmed that this fusion protein (BDNF-GFP) mimics the biological fu nctions and the release kinetics of unfused (native) BDNF. Second, when BDN F-GFP was expressed in cultured hippocampal neurons, we observed that this protein formed striking clusters in the neurites of mature neurons and colo calized with the PSD-95 immunoreactivity. Such a clustered BDNF-GFP rapidly disappeared in response to depolarization with KCl, as revealed by confoca l microscopic studies. These data suggest that BDNF is locally and rapidly released at synaptic sites in an activity-dependent manner. Optical studies using BDNF-GFP may provide important evidence regarding the participation of BDNF in synaptic plasticity. (C) 2001 Wiley-Liss, Inc.