Hydrogen peroxide-forming NADH oxidase belonging to the peroxiredoxin oxidoreductase family: Existence and physiological role in bacteria

Citation
Y. Nishiyama et al., Hydrogen peroxide-forming NADH oxidase belonging to the peroxiredoxin oxidoreductase family: Existence and physiological role in bacteria, J BACT, 183(8), 2001, pp. 2431-2438
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
183
Issue
8
Year of publication
2001
Pages
2431 - 2438
Database
ISI
SICI code
0021-9193(200104)183:8<2431:HPNOBT>2.0.ZU;2-2
Abstract
Amphibacillus xylanus and Sporolactobacillus inulinus NADH oxidases belongi ng to the peroxiredoxin oxidoreductase family show extremely high peroxide reductase activity for hydrogen peroxide and alkyl hydroperoxides in the pr esence of the small disulfide redox protein, AhpC (peroxiredoxin), In order to investigate the distribution of this enzyme system in bacteria, 15 bact erial strains were selected from typical aerobic, facultatively anaerobic, and anaerobic bacteria, AhpC-linked alkyl hydroperoxide reductase activitie s were detected in most of the tested strains, and especially high activiti es were shown in six bacterial species that grow well under aerobic conditi ons, including aerobic bacteria (Alcaligenes faecalis and Bacillus lichenif ormis) and facultatively anaerobic bacteria (Amphibacillus xylanus, Sporola ctobacillus inulinus, Escherichia coli, and Salmonella enterica serovar Typ himurium). In the absence of AhpC, the purified enzymes from A. xylanus and S. inulinus catalyze the NADH-linked reduction of oxygen to hydrogen perox ide. Similar activities were observed in the cell extracts from each of the se six strains. The cell extract of B. licheniformis revealed the highest A hpC-linked alkyl hydroperoxide reductase activity in the four strains, with V-max values for hydrogen peroxide and alkyl hydroperoxides being similar to those for the enzymes from A. xylanus and S. inulinus, Southern blot ana lysis of the three strains probed with the A. xylanus peroxiredoxin reducta se gene revealed single strong bands, which are presumably derived from the individual peroxiredoxin reductase genes, Single bands were also revealed in other strains which show high AhpC-linked reductase activities, suggesti ng that the NADH oxidases belonging to the peroxiredoxin oxidoreductase fam ily are widely distributed and possibly play an important role both in the peroxide scavenging systems and in an effective regeneration system for NAD in aerobically growing bacteria.