Ability for anaerobic growth is not sufficient for development of the petite phenotype in Saccharomyces kluyveri

Moller, K Olsson, L Piskur, J

K. Moller et al., Ability for anaerobic growth is not sufficient for development of the petite phenotype in Saccharomyces kluyveri, J BACT, 183(8), 2001, pp. 2485-2489

31

INGLESE

Article

Microbiology

JOURNAL OF BACTERIOLOGY

0021-9193 → ACNP

183

8

2001

2485 - 2489

ISI

0021-9193(200104)183:8<2485:AFAGIN>2.0.ZU;2-V

Saccharomyces cerevisiae is a petite-phenotype-positive ("petite-positive") yeast, which can successfully grow in the absence of oxygen. On the other hand, Kluyveromyces lactis as well as many other yeasts are petite negative and cannot grow anaerobically. In this paper, we show that Saccharomyces k luyveri can grow under anaerobic conditions, but while it can generate resp iration-deficient mutants, it cannot generate true petite mutants. From a p hylogenetic point of view, S. kluyveri is apparently more closely related t o S. cerevisiae than to K. lactis. These observations suggest that the prog enitor of the modern Saccharomyces and Kluyveromyces yeasts, as well as oth er related genera, was a petite-negative and aerobic yeast. Upon separation of the K. lactis and S. kluyveri-S. cerevisiae lineages, the latter develo ped the ability to grow anaerobically. However, while the S. kluyveri linea ge has remained petite negative, the lineage leading to the modern Saccharo myces sensu stricto and sensu late yeasts has developed the petite-positive characteristic.