EGF regulates a complex pattern of gene expression and represses smooth muscle differentiation during the neurotypic conversion of the neural-crest-derived TC-1S cell line

Citation
G. Giannini et al., EGF regulates a complex pattern of gene expression and represses smooth muscle differentiation during the neurotypic conversion of the neural-crest-derived TC-1S cell line, EXP CELL RE, 264(2), 2001, pp. 353-362
Citations number
52
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
EXPERIMENTAL CELL RESEARCH
ISSN journal
0014-4827 → ACNP
Volume
264
Issue
2
Year of publication
2001
Pages
353 - 362
Database
ISI
SICI code
0014-4827(20010401)264:2<353:ERACPO>2.0.ZU;2-8
Abstract
EGF, known to sustain CNS neuronal progenitors, also promotes a neurotypic response in the thymic neural-crest-derived TC-1S cell line. We report here the use of TC-1S cells as a model to identify the genetic programs regulat ed during the neurotypic response induced by EGF and to isolate 23 EGF-resp onsive genes. Among them 5 represent novel cDNAs, while 18 are known genes, whose regulation by EGF is associated with the mitogenic or differentiatin g effects of the growth factor. The repression of smooth muscle alpha -acti n and SM22 alpha genes by EGF and their increase by TGF beta suggest that t he TC-1S line includes neural crest multipotent cells whose smooth muscle d ifferentiation is repressed upon EGF treatment and stimulated by TGF beta. Therefore, we identified a complex pattern of EGF-target genes and propose EGF as a novel signal able to recruit postmigratory neural-crest-derived ce lls along proliferation and cell lineage choice pathways. (C) 2001 Academic Press.