Evaluation of insulin permeability and effects of absorption enhancers on its permeability by an in vitro pulmonary epithelial system using Xenopus pulmonary membrane

Citation
A. Yamamoto et al., Evaluation of insulin permeability and effects of absorption enhancers on its permeability by an in vitro pulmonary epithelial system using Xenopus pulmonary membrane, BIOL PHAR B, 24(4), 2001, pp. 385-389
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
0918-6158 → ACNP
Volume
24
Issue
4
Year of publication
2001
Pages
385 - 389
Database
ISI
SICI code
0918-6158(200104)24:4<385:EOIPAE>2.0.ZU;2-A
Abstract
The permeability of insulin across Xenopus pulmonary membrane and the effec ts of various absorption enhancers on Insulin permeability were examined us ing an in vitro Ussing chamber technique. Absorption enhancers used in this study were sodium caprate (NaCap), sodium glycocholate (NaGC), sodium sali cylate (NaSal) and ethylenediaminetetraacetic acid disodium salt (EDTA). Th e permeability of insulin across Xenopus Pulmonary membrane significantly i ncreased in the presence of NaCap and NaGC, while EDTA and NaSal did not en hance the permeability In addition, the enhancing effect of NaGC increased as the concentrations of these enhancers increased. Transmembrane resistanc e (Rm) of Xenopus lung was markedly decreased in the presence of these enha ncers, and NaCap showed a greater effect on Rm than NaGC. Furthermore, the amount of alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) releas ed from the apical side of the Xenopus pulmonary membrane increased in the presence of these enhancers. These results indicate that NaCap and NaGC imp rove the pulmonary absorption of insulin, but they are toxic to the pulmona ry membrane. These findings suggest that this method is useful for estimati ng the permeability characteristics of peptides across the pulmonary membra ne and for evaluating the effects of various additives on their permeabilit y and their membrane toxicity.