Interleukin-8 expression in AIDS-associated lymphoma B-cell lines

Citation
V. Sharma et L. Zhang, Interleukin-8 expression in AIDS-associated lymphoma B-cell lines, BIOC BIOP R, 282(2), 2001, pp. 369-375
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
282
Issue
2
Year of publication
2001
Pages
369 - 375
Database
ISI
SICI code
0006-291X(20010330)282:2<369:IEIALB>2.0.ZU;2-O
Abstract
Interleukin 8 (IL-8), a member of the CXC subfamily of chemokines, is a pot ent inflammatory cytokine produced by many cell types in response to severa l stimuli. In an attempt to determine whether human B-cell IL-8 functions a s an autocrine growth factor, a wide panel of B-cell lines derived from pat ients with AIDS-associated B-cell lymphomas (AABCL) (n = 5) and from non-AA BCLs (n = 8) was studied for expression of IL-8, IL-8 Receptor type A (IL-8 R), and secretion of IL-8 protein. Using RT-PCR and Northern Blot analysis, we were able to observe IL-8 expression ubiquitously. However, IL-8R expre ssion was seen only in EBV negative (4 out of 7) B-cell lines. EBV and HIV- 1 activated B-cell line; HBL-1, was the major secretor of IL-8. Our results demonstrate that IL-8 is expressed in malignant B-cell phenotypes that cor respond to a narrow window in the B-cell differentiation pathway (pre-B, ea rly-B, and intermediate-B) as well as in normal CD19-enriched B-cells. Furt hermore, IL-8 autocrine loops were not evident since IL-8R was detected onl y in cell lines that did not secrete IL-8 protein. (C) 2001 Academic Press.