Human umbilical vein endothelial cells (HUVEC) have previously been shown t
o synthesize the functional terminal pathway of complement based on the det
ection by radioimmunoassay of the terminal complement complex (TCC) on coin
cubated agarose beads. In addition, C7 secretion by these cells in amounts
comparable to C3, as well as C7 mRNA, has recently been demonstrated. Howev
er, it has not been possible to detect C5-6 and C8 in the fluid phase, and
only trace amounts of soluble C9. Against this background we examined wheth
er mRNA for the remaining terminal complement factors was present in HUVEC.
By the use of reverse transcription (RT)-polymerase chain reaction (PCR) a
nd Northern blot the presence of mRNA for complement factors C5, C6, C8 and
C9 was demonstrated.