Detection of mRNA for the terminal complement components C5, C6, C8 and C9in human umbilical vein endothelial cells in vitro

Citation
H. Langeggen et al., Detection of mRNA for the terminal complement components C5, C6, C8 and C9in human umbilical vein endothelial cells in vitro, APMIS, 109(1), 2001, pp. 73-78
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
APMIS
ISSN journal
0903-4641 → ACNP
Volume
109
Issue
1
Year of publication
2001
Pages
73 - 78
Database
ISI
SICI code
0903-4641(200101)109:1<73:DOMFTT>2.0.ZU;2-V
Abstract
Human umbilical vein endothelial cells (HUVEC) have previously been shown t o synthesize the functional terminal pathway of complement based on the det ection by radioimmunoassay of the terminal complement complex (TCC) on coin cubated agarose beads. In addition, C7 secretion by these cells in amounts comparable to C3, as well as C7 mRNA, has recently been demonstrated. Howev er, it has not been possible to detect C5-6 and C8 in the fluid phase, and only trace amounts of soluble C9. Against this background we examined wheth er mRNA for the remaining terminal complement factors was present in HUVEC. By the use of reverse transcription (RT)-polymerase chain reaction (PCR) a nd Northern blot the presence of mRNA for complement factors C5, C6, C8 and C9 was demonstrated.