Correlation of immunohistochemical p53 labeling index with inhibition ratein chemosensitivity test in gastric and colon cancer

Citation
N. Hosaka et al., Correlation of immunohistochemical p53 labeling index with inhibition ratein chemosensitivity test in gastric and colon cancer, ANTICANC R, 21(1A), 2001, pp. 229-235
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ANTICANCER RESEARCH
ISSN journal
0250-7005 → ACNP
Volume
21
Issue
1A
Year of publication
2001
Pages
229 - 235
Database
ISI
SICI code
0250-7005(200101/02)21:1A<229:COIPLI>2.0.ZU;2-S
Abstract
To determine whether the expression of p53, p21, bcl-2 or Ki-67 in cancer c ells is predictive of chemosensitivity immunohistochemical examination of t hese factors and chemosensitivity assays were performed on colon and gastri c cancer specimens. Chemosensitivity tests were performed using CDDP, 5-FU, MMC, ol ADR and inhibition rate (IR) was calculated by MTT assay. Before e xposure to anticancer drugs, the samples were investigated immunohistochemi cally for expression of the above factors and after anticancer drug exposur e by TUNNEL staining, for the presence of apoptotic cells. With 5-FU and MM C, the apoptotic index was well correlated with IR, so their effeets were r elated to apoptosis. Moreover, with these two agents, the p53 labeling inde x (LI) was inversely correlated with IR and p21-L1 showed a good correlatio n with IR. We therefore concluded that immunohistochemical studies for p53 and p21 were useful for predicting the chemosensitivities of colon and gast ric cancer to MMC and 5-FU.