Influence of restraint stress on the expression and the serine/threonine phosphatase activity of calcineurin in the rat brain

Citation
J. Takahashi et al., Influence of restraint stress on the expression and the serine/threonine phosphatase activity of calcineurin in the rat brain, SYNAPSE, 40(2), 2001, pp. 130-136
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
SYNAPSE
ISSN journal
0887-4476 → ACNP
Volume
40
Issue
2
Year of publication
2001
Pages
130 - 136
Database
ISI
SICI code
0887-4476(200105)40:2<130:IORSOT>2.0.ZU;2-K
Abstract
To investigate the role of calcineurin (CaN) in the pathogenesis of stress- related psychiatric disorders, we examined the expression of CaN A mRNA and the serine/threonine phosphatase activity of CaN in the rat brain followin g various restraint stress paradigms. Northern blot analysis revealed no si gnificant changes in the levels of CaN A mRNA expression, in either the fro ntal cortex or the hippocampus, in response to a single restraint stress fo r either 15, 45, or 90 min. In addition, no significant change in the level s of CaN A mRNA was found 3 or 6 h after a single restraint stress for 90 m in. In situ hybridization analysis revealed that a single restraint stress for 45 min had no influence on CaN A mRNA expression in the CA1 and CA3 pyr amidal cell layers or in the dentate gyrus granule cell layer of the hippoc ampus. However, serine/threonine phosphatase activity was significantly inc reased in both regions in response to a single restraint stress for either 45 or 90 min. These results demonstrate that the single restraint stress pa radigms used in this study increase the activity of CaN without any changes in the expression of CaN, suggesting that the activation of calmodulin and the increased levels of heat shock proteins in response to the restraint s tress may upregulate the activity of CaN in the rat brain. Synapse 40: 130- 136, 2001, (C) 2001 Wiley-Liss, Inc.