A new insertion sequence, IS231M, in an autoagglutinable isolate of Bacillus thuringiensis

Citation
Yc. Jung et al., A new insertion sequence, IS231M, in an autoagglutinable isolate of Bacillus thuringiensis, PLASMID, 45(2), 2001, pp. 114-121
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
PLASMID
ISSN journal
0147-619X → ACNP
Volume
45
Issue
2
Year of publication
2001
Pages
114 - 121
Database
ISI
SICI code
0147-619X(200103)45:2<114:ANISII>2.0.ZU;2-Y
Abstract
An insertion sequence was isolated from an autoagglutinable strain of Bacil lus thuringiensis. Analysis of its DNA sequence revealed high homology to t he IS231 family. The name IS231M is proposed for this new insertion sequenc e. IS231M is 1652 bp long and is delimited by two imperfect 20bp inverted r epeat sequences with two mismatches, which are flanked by two perfect I I-b p direct repeats (DRs). The region upstream of the open reading frame, pres umed to be able to form a stable hairpin structure, is particularly well co nserved in IS231M. Based on primary nucleotide sequences, IS231M is most ho mologous to IS231F and IS231G and most distant from IS231V and IS231W. Howe ver, as opposed to the single transposase A ORF found in IS131A. -B. -C. -D . -E and -G. IS231M has two overlapping open reading frames, OFR1 and ORF2, that could code for polypeptides of 334 and 143 amino acids, respectively Whether IS231M is a functional transposable element remains to be determine d. (C) 2001 Academic Press.