Sterol composition and growth of transgenic tobacco plants expressing type-1 and type-2 sterol methyltransferases

Citation
F. Sitbon et L. Jonsson, Sterol composition and growth of transgenic tobacco plants expressing type-1 and type-2 sterol methyltransferases, PLANTA, 212(4), 2001, pp. 568-572
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANTA
ISSN journal
0032-0935 → ACNP
Volume
212
Issue
4
Year of publication
2001
Pages
568 - 572
Database
ISI
SICI code
0032-0935(200103)212:4<568:SCAGOT>2.0.ZU;2-M
Abstract
Transgenic tobacco (Nicotiana tabacum L.) plants with altered sterol compos ition were generated by transformation with plant cDNAs encoding type-1 and type-2 sterol methyltransferases (SMTs; EC 2.1.1.41). For both SMT1 and SM T2 transformants, the transformation was associated with a reduction in the level of cholesterol, a non-alkylated sterol. In SMT1 transformants a corr esponding increase of alkylated sterols, mainly 24-methyl cholesterol, was observed. On the other hand, in SMT2 transformants the level of 24-methyl c holesterol was reduced, whereas the level of sitosterol was raised. No appr eciable alteration of total sterol content was observed for either genotype . The general phenotype of transformants was similar to that of controls, a lthough SMT2 transformants displayed a reduced height at anthesis. The resu lts show that plant sterol composition can be altered by transformation wit h an SMT1 cDNA without adverse effects on growth and development, and provi de evidence, in planta, that SMT1 acts at the initial step in sterol alkyla tion.