Quinolinic acid induces oxidative stress in rat brain synaptosomes

Citation
A. Santamaria et al., Quinolinic acid induces oxidative stress in rat brain synaptosomes, NEUROREPORT, 12(4), 2001, pp. 871-874
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROREPORT
ISSN journal
0959-4965 → ACNP
Volume
12
Issue
4
Year of publication
2001
Pages
871 - 874
Database
ISI
SICI code
0959-4965(20010326)12:4<871:QAIOSI>2.0.ZU;2-V
Abstract
The oxidative action of quinolinic acid (QUIN), and the protective effects of glutathione (GSH), and 2-amino-5-phosphonovaleric acid (APV), were teste d in rat brain synaptosomes, Reactive oxygen species (ROS) formation was qu antified after the exposure of synaptosomes to increasing concentrations of QUIN (25-500 muM) The potency of QUIN to induce lipid peroxidation (LP) wa s tested as a regional index of thiobarbituric acid-reactive substances (TB ARS) production, and the antioxidant actions of both GSH (50 muM) and APV ( 250 muM) on QUIN-induced LP were evaluated in synaptosomes prepared from di fferent brain regions. QUIN induced concentration-dependent increases in RO S formation and TBARS in all regions analyzed, but increased production of fluorescent peroxidized lipids only in the striatum and the hippocampus, wh ereas both GSH and APV decreased this index. These results suggest that the excitotoxic action of QUIN involves regional selectivity in the oxidative status of brain synaptosomes, and may be prevented by substances exhibiting antagonism at the NMDA receptor. NeuroReport 12:871-874 (C) 2001 Lippincot t Williams & Wilkins.