Global impact of sdiA amplification revealed by comprehensive gene expression profiling of Escherichia coli

Citation
Y. Wei et al., Global impact of sdiA amplification revealed by comprehensive gene expression profiling of Escherichia coli, J BACT, 183(7), 2001, pp. 2265-2272
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
0021-9193 → ACNP
Volume
183
Issue
7
Year of publication
2001
Pages
2265 - 2272
Database
ISI
SICI code
0021-9193(200104)183:7<2265:GIOSAR>2.0.ZU;2-A
Abstract
In Escherichia coli the amplification of sdiA, a positive activator of ftsQ AZ, genes that are essential for septation, results in mitomycin C resistan ce. To help us understand this resistance phenotype, genes whose expression was altered by increased sdiA dosage were identified using a DNA microarra y-based, comprehensive transcript profiling method. The expression of 62 ge nes was reduced by more than threefold; of these, 41 are involved in motili ty and chemotaxis, Moreover, the expression of 75 genes, 36 of which had be en previously characterized, was elevated at least threefold, As expected, increased sdiA dosage led to significantly elevated sdiA and 'ddlB-ftsQAZ-l pxC operon expression. Transcription of two genes, uvrY and uvrC, located d ownstream of sdiA and oriented in the same direction, was elevated about 10 -fold, although the intervening gene, yecF, of apposite polarity was unaffe cted by increased sdiA dosage, Three genes (mioC and gidAB) flanking the re plication origin, oriC, were transcribed more often when sdiA dosage was hi gh, as were 12 genes within 1 min of a terminus of replication, terB, Trans cription of the acrABDEF genes, mapping in three widely spaced loci, was el evated significantly, while several genes involved in DNA repair and replic ation (e.g., nei, recN, mioC, and mcrC) were moderately elevated in express ion. Such global analysis provides a link between septation and the respons e to DNA-damaging agents.