Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene

Citation
H. Dong et al., Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene, GENE, 264(2), 2001, pp. 187-196
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENE
ISSN journal
0378-1119 → ACNP
Volume
264
Issue
2
Year of publication
2001
Pages
187 - 196
Database
ISI
SICI code
0378-1119(20010221)264:2<187:GSAEAO>2.0.ZU;2-S
Abstract
Chromatin assembly factor-1 (CAF-1) plays essential roles in eukaryotic chr omatin assembly during DNA replication (Smith and Still man, 1989. Cell 58, 15-25), (Krude, 1999. Eur. J. Biochem. 263, 1-5). Its p150 subunit, involv ed in interaction with histone H3 and H4, is critical to the CAF-1 nucleoso me assembly activity. In this study, we sequenced a 96-kb genomic DNA regio n that includes a 42.8-kb CAF-1 p150 subunit gene (CHAFIA), and a 41.1-kb E EN gene. A scripted bioinformatics analysis pipeline (research agent) has b een set up to annotate the BAC sequence with a set of integrated algorithms . The CAF-1 p150 subunit gene contains 15 exons and 14 introns. The promote r region is characterized by deletional analyses, revealing a potential rep ressor. Tissue-correlated alternative splicing forms of the transcript was initially identified by EST clustering analysis, then confirmed by RT-PCR w hich resulted more splicing forms than computational prediction. (C) 2001 E lsevier Science B.V. All rights reserved.