Incidence of the cblA major subunit pilin gene amongst Burkholderia species

Citation
J. Richardson et al., Incidence of the cblA major subunit pilin gene amongst Burkholderia species, FEMS MICROB, 196(1), 2001, pp. 61-66
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
0378-1097 → ACNP
Volume
196
Issue
1
Year of publication
2001
Pages
61 - 66
Database
ISI
SICI code
0378-1097(20010301)196:1<61:IOTCMS>2.0.ZU;2-O
Abstract
PCR-amplification has been used to screen 75 isolates of the Burkholderia c epacia complex for the cblA pilin gene. PCR-amplified products of the corre ct size (664 bp) were cloned and sequenced and the sequences compared. Apar t from in the control, epidemic cystic fibrosis (CF)-associated B. cepacia lineage we also identified, for the first time, cblA genes in a unique, non -CF clinical isolate from France and a plant (onion) pathogenic isolate fro m Italy. The sequence of the cblA gene amplified from the clinical isolate was more diverged from the epidemic lineage than that amplified from the on ion pathogenic isolate. (C) 2001 Federation of European Microbiological Soc ieties. Published by Elsevier Science B.V. All rights reserved.