Environmental factors influencing the distribution of rRNA from Verrucomicrobia in soil

Citation
Dh. Buckley et Tm. Schmidt, Environmental factors influencing the distribution of rRNA from Verrucomicrobia in soil, FEMS MIC EC, 35(1), 2001, pp. 105-112
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
FEMS MICROBIOLOGY ECOLOGY
ISSN journal
0168-6496 → ACNP
Volume
35
Issue
1
Year of publication
2001
Pages
105 - 112
Database
ISI
SICI code
0168-6496(200103)35:1<105:EFITDO>2.0.ZU;2-Q
Abstract
The Verrucomicrobia constitute a newly discovered division of the Bacteria identified as a numerically abundant component of soil microbial communitie s in numerous sites around the world. The relative abundance of rRNA from V errucomicrobia was investigated in the soil to examine the influence of spe cific environmental factors on the distribution of Verrucomicrobia and to b etter understand the distribution of this group in terrestrial ecosystems. The abundance of the verrucomicrobial rRNA was determined by using a novel oligonucleotide probe that is specific for verrucomicrobial 16S rRNA. The a bundance of verrucomicrobial 16S rRNA in soil microbial communities was det ermined in relation to plant community composition and soil management hist ory over a period of 2 years. Additional samples were analyzed to determine if verrucomicrobial rRNA relative abundance changes in relation to either soil depth or soil moisture content. The Verrucomiurobia composed 1.9 +/- 0 .2% of the microbial community rRNA present in the 85 soil samples examined . The distribution of verrucomicrobial rRNA in the soil reveals that Verruc omicrobia art: significantly affected by environmental characteristics that change in relation to time, soil history, and soil depth, and reveals that a statistically significant amount of the variation in verrucomicrobial rR NA abundance can be explained by changes in soil moisture content. (C) 2001 Federation of European Microbiological Societies. published by Elsevier Sc ience B.V. All rights reserved.