Analysis of hypermutation of the 5' noncoding region in the BCL-6 gene

Citation
L. Wang et al., Analysis of hypermutation of the 5' noncoding region in the BCL-6 gene, CHIN MED J, 114(3), 2001, pp. 286-290
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
General & Internal Medicine
Journal title
CHINESE MEDICAL JOURNAL
ISSN journal
0366-6999 → ACNP
Volume
114
Issue
3
Year of publication
2001
Pages
286 - 290
Database
ISI
SICI code
0366-6999(200103)114:3<286:AOHOT5>2.0.ZU;2-#
Abstract
Objective To investigate BCL-6 gene mutations in Chinese populations with B -cell non-Hodgkin's lymphoma. Methods Polymerase chain reaction (PCR), denaturing gradient gel electropho resis (DGGE) and direct DNA sequencing were used to identify mutations in t he 5' noncoding region of the BCL-6 gene in a total of 40 cases of diffuse large-cell lymphoma (DLCL) and follicular lymphoma (FL). Results Nine cases were found to have base substitutions. The incidence of BCL-6 gene mutation and the frequency of single-base changes were approxima tely 25.7% and (0.56 - 1.10) x 10(-2)/bp, respectively. Conclusions The 5' regulatory region of the BCL-6 gene undergoes frequent s omatic hypermutation during lymphomagenesis and the identification of BCL-6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B-NHL.