Technetium-99m-tetrofosmin would be a substrate for multidrug resistance-associated protein (MRP): Comparison between a leukemia cell line with high MRP gene expression and its parental cell line

Citation
Xf. Li et al., Technetium-99m-tetrofosmin would be a substrate for multidrug resistance-associated protein (MRP): Comparison between a leukemia cell line with high MRP gene expression and its parental cell line, CANC BIO R, 16(1), 2001, pp. 17-23
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
ISSN journal
1084-9785 → ACNP
Volume
16
Issue
1
Year of publication
2001
Pages
17 - 23
Database
ISI
SICI code
1084-9785(200102)16:1<17:TWBASF>2.0.ZU;2-W
Abstract
The kinetics of cellular accumulation and retention of technetium-99m-tetro fosmin (Tc-99m-TF) were investigated in wild type HL60/WT cell line and in its doxorubicin-resistant HL60/DOX cell line with multidrug resistance-asso ciated protein (MRP), but without P-gp overexpression, to determine whether Tc-99m-TF is a substrate for MRP. Methods: The accumulation and washout of Tc-99m-TF were observed in both cell lines at 37 degreesC. The effect of v erapamil on the kinetics was also assessed. Results: Tc-99m-TF net accumula tion was significantly lower in HL60/DOX (1.35 +/- 0.23%) than in HL60/WT ( 12.79 +/- 0.47%) at 60 min (P < 0.001). Three minutes after exchanging the incubation solution to the tracer-free medium only 18.20 +/- 0.34% of Tc-99 m-TF remained in HL60/DOX, whereas 84.74 +/- 0.65% did in HL60/WT (P < 0.00 1). In the presence of 10 muM verapamil, Tc-99m-TF net accumulation in HL60 /DOX was 302% of the control and the washout was significantly delayed. Con clusion: Tc-99m-TF would be a substrate for MRP and Tc-99m-TF may be used a s a functional imaging agent of MRP in vivo.