QTLs for grain dry milling properties, composition and vitreousness in maize recombinant inbred lines

Citation
M. Sene et al., QTLs for grain dry milling properties, composition and vitreousness in maize recombinant inbred lines, THEOR A GEN, 102(4), 2001, pp. 591-599
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
THEORETICAL AND APPLIED GENETICS
ISSN journal
0040-5752 → ACNP
Volume
102
Issue
4
Year of publication
2001
Pages
591 - 599
Database
ISI
SICI code
0040-5752(200103)102:4<591:QFGDMP>2.0.ZU;2-4
Abstract
Vitreousness and kernel hardness are important properties for maize process ing and end-product quality. In order to examine the genetic basis of these traits, a recombinant inbred line population resulting from a cross betwee n a flint line (F-2) and a semident line (Io) was used to search for vitreo usness and kernel composition QTLs. Vitreousness was measured by image proc essing from a kernel section, while NIR spectroscopy was used to estimate s tarch, protein, cellulose, lipid and semolina yield. In addition, thousand- grain weight and grain weight per ear were measured. The MQTL method was us ed to map the QTLs for the different traits. An additional program allowed for the detection of interaction QTLs between markers. The total number of main-effect and interaction QTLs was similar. The QTLs were not evenly dist ributed but tended to cluster. Such clusters, mixing main-effect and intera ction QTLs, were observed at six positions : on chromosomes 1, 2, 3, 6, 8 a nd 9. Two of them, on chromosomes 6 and 9, concerned both QTLs for kernel-w eight traits and QTLs for kernel-composition traits (protein and cellulose) . Technological-trait QTLs (vitreousness or semolina yield) were located le ss than 16 cM from a protein-content QTL on chromosome 2, and were co-locat ed with lipid- and starch-content QTLs on chromosome 8. The co-location of a vitreousness and a semolina-yield QTL at the telomeric end of the chromos ome 2 (Bin 2.02) is likely to be meaningful since measurement of these rela ted traits, made by completely different methods (NIRS vs image processing) , yielded very close QTLs. A similar location was previously reported indep endently for a kernel-friability QTL. Comparing the map location of the num erous loci for known-function genes it was shown that three zein loci were closely linked to QTLs for vitreousness on chromosome 3, for semolina yield and starch on chromosome 4, and for protein, cellulose and grain weight on chromosome 9. Some other candidate genes linked to starch precursor metabo lism were also suggested on chromosomes 6 and 8.