Constitutive expression of human coagulating factor IX in HeLa cells by homologous recombination of the promoter

Citation
Xq. Yang et al., Constitutive expression of human coagulating factor IX in HeLa cells by homologous recombination of the promoter, SCI CHINA C, 44(1), 2001, pp. 18-24
Citations number
7
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN journal
1006-9305 → ACNP
Volume
44
Issue
1
Year of publication
2001
Pages
18 - 24
Database
ISI
SICI code
1006-9305(200102)44:1<18:CEOHCF>2.0.ZU;2-I
Abstract
Constitutive expression of hFIX protein in nonhepatocytes was studied. The gene targeting vector was constructed and transferred into HeLa cells. With the detection system of PCR, we demonstrated that the endogenous hFIX prom oter was replaced with an hCMV promoter when targeted insertion of the cons tructor was directed by the sequence homology. The expression of hFIX in th e modified HeLa cells, 11.2 ng/10(6) cell/24 h, strongly suggested that hFI X gene could be activated by a powerful promoter in nonhepatocytes. The res ults would make it possible to examine the feasibility of re-regulate gene expression by promoter replacement.