In vivo and in vitro diagnosis of Mycobacterium bovis infection

Authors
Citation
Lg. Adams, In vivo and in vitro diagnosis of Mycobacterium bovis infection, REV SCI TEC, 20(1), 2001, pp. 304-324
Citations number
151
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
REVUE SCIENTIFIQUE ET TECHNIQUE DE L OFFICE INTERNATIONAL DES EPIZOOTIES
ISSN journal
0253-1933 → ACNP
Volume
20
Issue
1
Year of publication
2001
Pages
304 - 324
Database
ISI
SICI code
0253-1933(200104)20:1<304:IVAIVD>2.0.ZU;2-4
Abstract
The intradermal delayed type hypersensitivity (DTH) skin test, using purifi ed protein derivative from culture of M. bovis or M, avium, is the most fre quently used test for diagnosis of tuberculosis or detection of M. bovis in fection in cattle. Many improvements have been made to the original tubercu lin test, and molecular approaches to identify and clone antigens may lead to improved specificity and sensitivity of DTH skin tests. Recent advances in technology have allowed the development of new in vitro techniques, such as antibody-based, cell-mediated immunity-based and nucleic acid-based dia gnostics, which allow more rapid diagnosis than bacteriological culture. Th e choice of diagnostic technique should consider both the population being investigated (e.g. apparently healthy animals or a herd with a high prevale nce of clinical infection) and the aim of the testing (e.g. the screening o f healthy animals or confirmation of infection in animals strongly suspecte d to be infected). Moreover, any evaluation of a diagnostic test must use a carefully selected control population which is representative of the popul ation to be tested in terms of relative proportions of infected and non-inf ected animals.