Apoptotic bodies in the cerebellum of Japanese patients with Creutzfeldt-Jakob disease

Citation
T. Kawashima et al., Apoptotic bodies in the cerebellum of Japanese patients with Creutzfeldt-Jakob disease, PATHOL INT, 51(3), 2001, pp. 140-144
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
PATHOLOGY INTERNATIONAL
ISSN journal
1320-5463 → ACNP
Volume
51
Issue
3
Year of publication
2001
Pages
140 - 144
Database
ISI
SICI code
1320-5463(200103)51:3<140:ABITCO>2.0.ZU;2-V
Abstract
The aim of this study was to provide morphological evidence for neuronal ap optosis in Creutzfeldt-Jakob disease (CJD) using hematoxylin-eosin (HE)-sta ined specimens. A microscopic examination showed typical apoptotic bodies w ere found in the granular layer of the cerebellum in 13 of 14 Japanese pati ents with CJD, while no apoptotic bodies were observed in any other areas o f the examined CJD brains. Most of the fragmented nuclei of the apoptotic c ells were labeled by in situ end-labeling of fragmented DNA, terminal deoxy nucleotidyl transferase-mediated dUTP-biotin nick-end labeling. To the auth ors' knowledge, this is the first report demonstrating neuronal apoptotic b odies in the human neurodegenerative disorders on HE-stained sections. The present findings indicate that apoptosis plays a major role in the neuronal loss of the cerebellar granule cells in CJD.