Serum antibody response induced in mice after oral administration of threedifferent antigens of enterotoxigenic Escherichia coli in enteric coated microparticles

Citation
Ja. Adachi et al., Serum antibody response induced in mice after oral administration of threedifferent antigens of enterotoxigenic Escherichia coli in enteric coated microparticles, J TRAVEL M, 7(6), 2000, pp. 304-308
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
General & Internal Medicine
Journal title
JOURNAL OF TRAVEL MEDICINE
ISSN journal
1195-1982 → ACNP
Volume
7
Issue
6
Year of publication
2000
Pages
304 - 308
Database
ISI
SICI code
1195-1982(200011/12)7:6<304:SARIIM>2.0.ZU;2-7
Abstract
Background: Gastric digestion of these antigens plays an important role, de creasing the ability to deliver antigens to the gut-associated lymphoid tis sue. To overcome this obstacle, microencapsulated antigens from enterotoxig enic Escherichia coil (ETEC) were evaluated for oral immunization of mice. Methods: Four groups of 10 each received 3 series of 3 doses each of (1) B subunit of cholera toxin (CTB), similar to heat-labile toxin of ETEC, (2) f ormalin-killed whole cell ETEC H10407 (FK-ETEC), (3) crude preparation of c olonization factor antigen I (CFA/1), or (4) placebo. Serum antibody was me asured on day 0 and 60 by ELISA. Results: In group 1 a CTB antibody response was induced in all mice, 3 with 1:10(5) titer and 7 with 1:10(6). These antibodies neutralized cholera tox in-induced steriodogenesis of Y-1 adrenal cells. In group 2, 8 mice develop ed a whole H10407 bacteria antibody titer of 1:100, one 1:200 and one showe d no immune response. In the same group, an anti-CFA/I response was observe d in 6 mice and anti-LPS in 4 mice as determined by Western blot. All mice in group 3 showed > 1:10(4) anti-CFA/I antibody titer. Group 4 mice did not develop an immune response to any ETEC antigens. Conclusion: Microencapsulation appears to be a suitable approach for oral v accination against ETEC and Vibrio cholerae.