O. Kanauchi et al., Dietary fiber fraction of germinated barley foodstuff attenuated mucosal damage and diarrhea, and accelerated the repair of the colonic mucosa in an experimental colitis, J GASTR HEP, 16(2), 2001, pp. 160-168
Background and Aims. Germinated barley foodstuff (GBF) contains protein and
insoluble dietary fiber. We have previously shown in ulcerative colitis pa
tients and a colitis model that GBF feeding attenuates mucosal damage by in
creasing luminal butyrate levels. However, the detailed mechanism remains u
nclear because of its heterogeneous nature. The present study was carried o
ut to: (i) evaluate the active ingredient in GBF; and (ii) examine its effe
ct on the repair process in colonic inflammation by using a dextran sulfate
sodium (DSS) colitis model.
Methods: Colitis was induced by feeding a diet containing 0.5-3.5% DSS to m
ale Sprague-Dawley rats. (i) Active ingredient: GBF was fractionated enzyma
tically into fiber- and protein-rich fractions. Each fraction was administe
red to DSS-colitis rats. Clinical signs, cecal short chain fatty acid conce
ntrations and serum alpha (1)-acid glycoprotein (AAG) levels were determine
d. (ii) Effect on mucosal repair: GBF with or without salazosulfapyridine (
SASP), or SASP alone was administered to rats after the onset of colitis. S
even days after initial treatment, the number of epithelial cells in HE sec
tions was evaluated morphologically in a blind fashion and serum AAG was de
Results: (i) Germinate barley foodstuff and GBF-fiber significantly attenua
ted the clinical signs of colitis and decreased serum AAG levels, with a si
gnificant increase in cecal butyrate production, while GBF-protein did not.
(ii) Treatment with GBF alone and GBF plus SASP significantly accelerated
colonic epithelial repair and improved clinical signs.
Conclusions: These findings suggest that the fiber fraction of GBF may effe
ctively enhance luminal butyrate production, and thereby accelerate colonic
epithelial repair in colitis. (C) 2001 Blackwell Science Asia Pty Ltd.