Budding yeast chromosome structure and dynamics during mitosis

Citation
Cg. Pearson et al., Budding yeast chromosome structure and dynamics during mitosis, J CELL BIOL, 152(6), 2001, pp. 1255-1266
Citations number
47
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
152
Issue
6
Year of publication
2001
Pages
1255 - 1266
Database
ISI
SICI code
0021-9525(20010319)152:6<1255:BYCSAD>2.0.ZU;2-Q
Abstract
Using green fluorescent protein probes and rapid acquisition of high-resolu tion fluorescence images, sister centromeres in budding yeast are found to be separated and oscillate between spindle poles before anaphase B spindle elongation. The rates of movement during these oscillations are similar to those of microtubule plus end dynamics. The degree of preanaphase separatio n varies widely, with infrequent centromere reassociations observed before anaphase. Centromeres are in a metaphase-like conformation, whereas chromos ome arms are neither aligned nor separated before anaphase, Upon spindle el ongation, centromere to pole movement (anaphase A) was synchronous for all centromeres and occurred coincident with or immediately after spindle pole separation (anaphase B). Chromatin proximal to the centromere is stretched poleward before and during anaphase onset. The stretched chromatin was obse rved to segregate to the spindle pole bodies at rates greater than centrome re to pole movement, indicative of rapid elastic recoil between the chromos ome arm and the centromere. These results indicate that the elastic propert ies of DNA play an as of yet undiscovered role in the poleward movement of chromosome arms.