Sema4C, a transmembrane semaphorin, interacts with a post-synaptic densityprotein, PSD-95

Citation
S. Inagaki et al., Sema4C, a transmembrane semaphorin, interacts with a post-synaptic densityprotein, PSD-95, J BIOL CHEM, 276(12), 2001, pp. 9174-9181
Citations number
52
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
0021-9258 → ACNP
Volume
276
Issue
12
Year of publication
2001
Pages
9174 - 9181
Database
ISI
SICI code
0021-9258(20010323)276:12<9174:SATSIW>2.0.ZU;2-S
Abstract
Semaphorins are known to act as chemorepulsive molecules that guide axons d uring neural development. Sema4C, a group 4 semaphorin, is a transmembrane semaphorin of unknown function. The cytoplasmic domain of Sema4C contains a proline-rich region that may interact with some signaling proteins. In thi s study, we demonstrate that Sema4C is enriched in the adult mouse brain an d associated with PSD-95 isoforms containing PDZ (PSD-95/DLG/ZO-1) domains, such as PSD-95/SAP90, PSD-93/chapsin110, and SAP97/DLG-1, which are concen trated in the post-synaptic density of the brain. In the neocortex, S4C is enriched in the synaptic vesicle fraction and Triton X-100 insoluble post-s ynaptic density fraction. Immunostaining for Sema4C overlaps that for PSD-9 5 in superficial layers I-IV of the neocortex. In neocortical culture, S4C is colocalized with PSD-95 in neurons, with a dot-like pattern along the ne urites. Sema4C thus may function in the cortical neurons as a hi-directiona l transmembrane ligand through interacting with PSD-95.