The hepatitis C virus (HCV) induces a long-term increase of interleukin-10production by human CD4(+) T cells (H9)

Citation
O. Delpuech et al., The hepatitis C virus (HCV) induces a long-term increase of interleukin-10production by human CD4(+) T cells (H9), EUR CYTOKIN, 12(1), 2001, pp. 69-77
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
EUROPEAN CYTOKINE NETWORK
ISSN journal
1148-5493 → ACNP
Volume
12
Issue
1
Year of publication
2001
Pages
69 - 77
Database
ISI
SICI code
1148-5493(200103)12:1<69:THCV(I>2.0.ZU;2-F
Abstract
Patients with chronic hepatitis C present an imbalance of Th1/Th2 cytokine production. Therefore, we investigated whether the exposure of the CD4(+) T cell line H9 to HCV could induce activation of cells through synthesis of IL-10. Three infection protocols were performed to enhance HCV propagation. Viral particles were prepared by ultracentrifugation of serum from patients. From 3 to 81 days post-infection (p,i.), HCV-RNA was monitored both in supernat ants and cells by nested RT-PCR, IL-10 protein in medium by ELISA, and IL-1 0 mRNA in cells by semi-quantitative RT-PCR, The expression of tetraspanins was analyzed by flow cytometry, The PKC signal pathway was studied using s pecific inhibitors. The H9 cells express CD81, HCV-RNA (+) was detected in cells until 21 days p,i, and in culture media over 39 days p,i, Up to day 8 1 p,i,, HCV exposure induced a specific, 2-fold increase of IL-10 productio n by H9 cells. IL-IO production was inhibited by a PKC inhibitor (Calphosti n C), This study shows that even if the infection of H9 T cells did not res ult in any viral progeny, HCV induced the activation of IL-10 secretion, wh ich supports the role of IL-10 in HCV pathogenesis.