Bio-markers for egg quality determination in cyprinid fish

Citation
F. Lahnsteiner et al., Bio-markers for egg quality determination in cyprinid fish, AQUACULTURE, 195(3-4), 2001, pp. 331-352
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Aquatic Sciences
Journal title
AQUACULTURE
ISSN journal
0044-8486 → ACNP
Volume
195
Issue
3-4
Year of publication
2001
Pages
331 - 352
Database
ISI
SICI code
0044-8486(20010416)195:3-4<331:BFEQDI>2.0.ZU;2-G
Abstract
The present study investigated potential bio-markers (ovarian fluid paramet ers, egg weight and weight increase during water hardening, biochemical egg composition, egg enzyme activities) for egg quality determination in the c ommon carp (Cyprinus carpio), the silver carp (Hypophthalmichthys molitrix) , the grass carp (Ctenopharyngodon idella) and the bleak (Chalcalburnus cha lcoides). For all investigated species, the study revealed highly significant correla tions between the egg fertilization rate and the weight of water-hardened e ggs, the percent weight increase during water hardening and the ovarian flu id pH, protein concentration, and aspartate aminotransferase activity. The fertilization rate was further correlated with the activities of malate deh ydrogenase and pyruvate kinase of the eggs, and in C. calpio and Cha. chalc oides with the respiration activity, too. Investigated biochemical paramete rs of the eggs (protein, peptides, fructose, galactose, glucose, non-esteri fied fatty acids, esterified fatty acids, total DNA and RNA) were not corre lated with the fertilization rate. The possible use of the analysed parameters for prediction of egg quality d uring short-term storage was also investigated in C. carpio and Cte. idella : during short-term storage for 4 h at 4 degreesC, the fertilization rate s ignificantly decreased. Also, the weight of the eggs after water hardening, the percent weight increase due to water hardening and the ovarian fluid p H and ovarian fluid aspartate aminotransferase changed in comparison to fre sh samples and were highly significantly correlated with the fertilization rate. In contrast, the biochemical composition of the eggs (protein, peptid es, fructose, galactose, glucose, non-esterified fatty acids, esterified fa ttyacids, total DNA and RNA) and egg enzyme activities (phosphofructokinase , pyruvate kinase, protease, lipase, NAD-dependent malate dehydrogenase, re spiration rate, NADP-dependent isocitrate dehydrogenase, aspartate aminotra nsferase) remained constant. (C) 2001 Elsevier Science B.V. All rights rese rved.